Inducible nitric oxide synthase, nitrotyrosine and apoptosis in gastric adenocarcinomas and their correlation with a poor survival

Figure 1 iNOS staining. A: iNOS staining detected in cytoplasms of tumor cells (thick arrow) and in inflammatory cells (thin arrows); B: Negative control; C: No positive immunohistostaining for iNOS in normal gastric tissue sections. Magnification A and B ×1000; C ×400.

Figure 2 NT staining. A: NT staining detected in cytoplasms and nuclei of tumor cells (arrow); B: Negative control; C: No positive immunoreactivity in normal gastric tissue sections. Magnification ×400.

Figure 3 Apoptotic cells and bodies staining. A: An apoptotic body characterized by a pyknotic nucleus surrounded by shrunken cytoplasm and separated from the surrounding cells by a halo (arrow); B: Negative control. Magnification A ×1000; B ×400.

Figure 4 Significantly direct correlation between AI and NT expression observed by using the Spearman rank correlation coefficient (r = 0. 718; P<0.001).

Figure 5 Survival of patients relative to iNOS status. Solid line: negative or low iNOS expression; dotted line: intermediate or high iNOS expression.