Basic Research
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Apr 21, 2005; 11(15): 2306-2312
Published online Apr 21, 2005. doi: 10.3748/wjg.v11.i15.2306
Figure 1
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Figure 1 Maximum cumulative concentration-response curves to (A) phenylephrine (PEP) (10-10 to 10-4 mol/L), (B) AVP (10-10 to 10-4 mol/L) in thoracic aorta from CBL (◆) and sham (□) rats. aP<0.05 vs sham rats (n = 12 in each group with different agonist stimulation). Sham: sham-operated rats; CBL: common bile duct-ligated rats.
Figure 2
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Figure 2 Maximum cumulative concentration-response curves to (A) synthetic TXA2 analog U-46619 (10-9 to 10-5 mol/L), (B) receptor-independent G-protein stimulus NaF (10-3-1 mol/L)/AlCl3 (30 μmol/L), (C) direct activation of protein kinase C by PdBU (10-8 to 3×10-5 mol/L) in thoracic aorta from CBL (◆) and sham-operated (□) rats, n = 10 in each group with different agonist stimulation.
Figure 3
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Figure 3 (A) Phenylephrine (PEP) (10-7 to 10-5 mol/L) and (B) AVP (10-7 to 10-5 mol/L)-induced [3H] IP3 formation in the aortic ring from CBL (black bars) and sham (white bars) rats. [3H] IP3 formation was expressed as the percentage of counts (cpm) per minute in the presence of agonist divided by the counts without agonist (basal formation). The data are expressed as mean±SE. aP<0.05 vs sham rats, n = 8 in each group with different agonist stimulation. Sham: sham-operated rats; CBL: common bile duct-ligated rats.
Figure 4
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Figure 4 (A) U-46619, a synthetic TXA2 analog (10-7 and 10-6 mol/L), (B) NaF/AlCl3 (0. 1 and 1 mol/L)-induced [3H] IP3 formation in the aortic ring from CBL (black bars) and sham (white bars) rats. [3H] IP3 formation was expressed as the percentage of counts (cpm) per minute in the presence of agonist divided by the counts without agonist (basal formation). The data are expressed as mean±SE, n = 8 in each group with different agonist stimulation.
Figure 5
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Figure 5 (A) Maximum cumulative concentration-response curves to phenylephrine (PEP) (10-10 to 10-4 mol/L) in thoracic aorta from CBL (◆) and sham (□) rats with and without preincubation with L-NIL (L-N(6)-(1-iminoethyl)-lysine, a selective iNOS inhibitor). n = 10 in each group with different agonist stimulation. CBL: common bile duct-ligated rats; sham: sham-operated rats. (B) Western blot analysis of inducible nitric oxide synthase (iNOS) protein expression from thoracic aorta in CDL and sham-operated rats. Lanes A and B: CBL rats; lanes C and D: sham rats.