Expression of intestinal trefoil factor, proliferating cell nuclear antigen and histological changes in intestine of rats after intrauterine asphyxia

Figure 1 ITF mRNA expression in intestine after intrauterine asphyxia detected by RT-PCR. Lane 1: after intrauterine asphyxia 0-h group; lane 2: control group; lane 3: after intrauterine asphyxia 24-h group; lane 4: control group; lane 5: after intrauterine asphyxia 48-h group; lane 6: control group; lane 7: after intrauterine asphyxia 72-h group; lane 8: control group.

Figure 2 Immunohistochemical results of PCNA (400×). A: Positive staining of intestinal mucosal goblet cell nuclei; B: Decline of PCNA positive staining 48 h after intrauterine asphyxia.

Figure 3 Intestinal tissue HE staining (400×). A: Mature intestinal tissue in normal newborn rats; B: Obvious structural changes, denuded villi with lamina propria and exposed dilated capillaries increased cellularity of lamina, declined quantity of villi 48 h after intrauterine asphyxia.