Expression of p16 gene and Rb protein in gastric carcinoma and their clinicopathological significance

Figure 1 Immunohistochemical staining of P16 protein expression. Arrow shows positive cell. A: Normal gastric mucosa SP ×400; B: dysplastic gastric mucosa SP ×400; C: well-differentiated adenocarcinoma SP ×400; D: poorly-differentiated adenocarcinoma SP ×400; E: undifferentiated carcinoma SP ×200; F: undifferentiated carcinoma SP ×400.

Figure 2 PCR amplification products in exon 2 of p16 gene. Lanes 1-3, 5-6, 8-10: GC; Lane 4: normal gastric mucosa; Lane 7: cancer adjacent tissue; Lane M: marker; Few PCR products in lane 3 and no PCR product in lane 5.

Figure 3 Exon 2 of p16 gene analyzed by SSCP, Lanes 2, 3, 5, 6, 7: GC; Lane 4: cancer adjacent tissue; Lane 1: normal gastric mucosa. No electrophoresis band in lane 3; weak electrophoresis band in lane 5; no abnormal electrophoresis band in all lanes.

Figure 4 Rb protein expression in undifferentiated carcinoma, SP×200.