Hepatic preconditioning of doxorubicin in stop-flow chemotherapy: NF-κB/IκB-α pathway and expression of HSP72

Figure 1 Western blot analysis of HSP72 (A) and NF-κB p65 subunit (B) in liver cytoplasmic extracts before SFC in NS and DOX groups.

Figure 2 Western blot analysis of NF-κB presentation (A) in liver nuclear extracts, IκB-α expression (B) and immunoprecipitation/Western blot analysis of p-IκB-α expression (C) in liver cytoplasmic extracts at different time points, i. e. before SFC, at the end of SFC (20 min), 30 min after the restoration of the abdominal circulation, 3 h after the restoration of the abdominal circulation, 6 h after the restoration of the abdominal circulation. WB, Western blot, IP, Immunoprecipitation.

Figure 3 Semiquantitive RT-PCR analysis of β-actin (A) as an internal control and TNF-α mRNA (B) expression in liver tissues at different time points, i. e., before SFC, at the end of SFC (20 min), 30 min after the restoration of the abdominal circulation, 3 h after the restoration of the abdominal circulation, 6 h after the restoration of the abdominal circulation, and the ratio between the expression of TNF-α mRNA at different time points and the expression of TNF-α mRNA (C).

Figure 4 Serum level of ALT (A) and AST (B) at different time points before and after SFC respectively, 0, before SFC, I20 min, at the end of SFC (20 min), R3h, 3 h after the restoration of the abdominal circulation, R6 h, 6 h after the restoration of the abdominal circulation, R24 h, 24 h after the restoration of the abdominal circulation, R48 h, 48 h after the restoration of the abdominal circulation, R7d, 7 d after the restoration of the abdominal circulation.