Chromic-P32 phosphate treatment of implanted pancreatic carcinoma: Mechanism involved

Figure 1 Effective half-life in ³²P colloids 7. 4 and 14.8 MBq injected group averaged 13 d.

Figure 2 Results of immuno-histochemistry assay of CD34 expression in Pc-3. A: Plenty of stained and densely arranged micro-vessels in tumor field (arrow); B: After medication the tumor cells loosely arranged in tumor field with scanty of micro-vessels stained or no micro-vessels (SABC ×400); PCNA expression in Pc-3. C: In control site, tumor cells were densely arranged with poly nuclei stained in deep brown yellow; D: In each of the dosed sites loosely arranged tumor cells merely with few nuclei stained in light brown yellow color (SABC ×400).

Figure 3 TEM shows the changes in Pc-3 tumor cell; A: Tumor cells (arrow) in control group grew vividly with large nuclei and some nucleoli (12×10³×1. 2); B: Tumor cells (arrow) were damaged, only the contour of nucleus remained (6×10³); C: A few remaining tumor cells arranged as a glandular lumen (arrow) with small nucleoli (8×10³×1.2); D: At low dose point, well differentiated Pc-3 cell with small nucleoli, plenty of euchromatin, many long micro-villi on cell surface (arrow), glandular lumens interplaced between the cells having the tendency of exocrine formation (15×10³×1.2).

Figure 4 Transmission electron microscopy of ILN A: Metastatic Pc-3 cells (arrow) in lymphatic tissue of control group (4×10³); B: After ³²P colloids irradiation apoptotic cells (arrow) in the connective tissue and endothelium of lymphatic sinuses (2. 5×10³); C: Denatured tumor cells (arrow) after medication (5×10³×1.2); D: ILN under light microscope: lymphatic tissue recovered to normal 4 wk after medication (HE ×400).

Figure 5 Results of TEM: A: Cell necrosis, breaking into debris (arrow) (10×10³); B: Early apoptosis (arrow). Chromatin aggregated at periphery (10×10³×1.2); C: Medium stage of apoptosis (arrow) showing nucleus shrunk and broken (10×10³×1.2).