Hypermethylation of CpG island in O6-methylguanine-DNA methyltransferase gene was associated with K-ras G to A mutation in colorectal tumor

Figure 1 Methylation-specific PCR result of MGMT in colorectal tumors and normal colorectal mucosa. Colorectal carcinomas (45 °C) showed both unmethylated (U) and methylated (M) bands, but normal tissues (N) showed only unmethylated bands.

Figure 2 MGMT expressed in the nuclei of normal colorectal mucosa cells.

Figure 3 Loss of MGMT expression in colorectal carcinomas.

Figure 4 MASA-PCR products for detection of K-ras point mutations in colorectal tumors. Set 1 and set 2 showed the first and second G to A mutation in K-ras codon 12 (GGT), respectively.