Inhibitory effect of tumor suppressor p33ING1b and its synergy with p53 gene in hepatocellular carcinoma

Figure 1 FACS analysis of cell apoptosis in three hepatoma cell lines (A-C) and arrest of HepG2 cells at G₀/G₁ cell cycle (D-G).

Figure 2 Growth curves for HepG2 cells stably transfected with pcDNA3 (▲), pcDNA3-p33^ING1b (●), or pcDNA3-α p33^ING1b (■) in complete medium containing 10% serum.

Figure 3 Analysis of p21^WAF1/CIP1 promoter activation in three hepatoma cell lines.

Figure 4 IHC staining for p33^ING1b on HCC (A), para-cancerous tissues (B), and normal liver tissues (C), and for p53 on HCC (D). A and D: p33^ING1b and p53 were nuclear-positive and located in the same area of HCC, ×400; B and C: p33^ING1b presented weak IHC staining, ×200.

Figure 5 Altered migration pattern of PCR products of p33^ING1b gene from HCC genomic DNA by SSCP. N, normal DNA; T, tumor DNA; S, para-cancerous tissue DNA.