Establishment of a P-glycoprotein substrate screening model and its preliminary application

Figure 1 Analysis of pcDNA3. 1(+)/MDR1 digested by EcoRI. M: DNA ladder; A: pcDNA3.1(+)/MDR1 digested by EcoRI.

Figure 2 Map of real-time fluorescent quantitative RT-PCR. Blue curve: Bcap37; Other color curves: Bcap37 cell clones trans-fected with pcDNA3.1(+)/MDR1; Red curve stands for the clone expressing higher P-gp level than others.

Figure 3 Western blot analysis of P-gp expression in Bcap37 and Bcap37/MDR1 cells. A: Bcap37; B, C, D, E: Bcap37 cell clones transfected with pcDNA3.1(+)/MDR1.

Figure 4 P-gp immunohistochemical staining in Bcap37 (A) and Bcap37/MDR1 (B) cells.

Figure 5 Amount of quercetin accumulation in cells with/without verapamil.