1α, 25-dihydroxyvitamin D3 prevents DNA damage and restores antioxidant enzymes in rat hepatocarcinogenesis induced by diethylnitrosamine and promoted by phenobarbital

Figure 1 Basic experimental protocol.

Figure 4 Shows the section of the rat liver (group A) showing abnormal hepatic architecture after a single i. p. Injection of DEN (200 mg/kg b.m.) (HE × 325).

Figure 5 Shows the thin section of normal rat liver untreated (group B) showing hepatocellular architecture (HE × 325).

Figure 6 Shows section of rat liver (group C) initiated with DEN and supplemented with 1α, 25(OH)₂D₃ [0. 3 μg/100 μL propylene glycol per os twice a week for 20 wk] showing almost normal hepatic architecture (HE × 325).

Figure 2 Box-and-whisker plot of the distribution of DNA damage treated with 1alpha, 25(OH)₂D₃ in DEN induced rat hepatocarcinogenesis promoted by phenobarbital.

Figure 3 Tailed cells (%) in hepatic cells of rats treated with 1alpha, 25(OH)₂D₃ in DEN induced rat hepatocarcinogenesis promoted by phenobarbital.