Effect of cold-ischemia time on nuclear factor-κB activation and inflammatory response in graft after orthotopic liver transplantation in rats

Figure 1 Changes in serum ALT levels after reperfusion. Serum ALT levels significantly increased in the 24 h (closed square) and 18 h (closed circle) cold-preserved groups, compared with the 6 h group (closed triangle) within 0.5 h after reperfusion (P < 0.05, respectively). Results are mean ± SD for each time point in each group (n = 6).

Figure 2 Changes in p65/relA subunit levels after reperfusion. P65/relA subunit levels significantly increased in the 24 h (closed square) and 18 h (closed circle) cold-preserved groups, compared with the 6 h group (closed triangle) at 0.5 h, 1 h, and 2 h after reperfusion (P < 0.05, respectively). Results are mean ± SD for each time point in each group (n = 6).

Figure 3 Changes in intrahepatic inflammatory mediator responses after reperfusion. A: TNF-α levels, B: CINC levels, C: ICAM-1 levels. TNF-α levels significantly increased in the 24 h and 18 h groups 0.5 h after reperfusion, compared with the 6 h group (P < 0.05, respectively). CINC levels significantly in-creased in the 24 h and 18 h groups 2 h after reperfusion, com-pared with the 6 h group (P < 0.05, respectively). ICAM-1 levels significantly increased in the 24 h and 18 h cold-preserved groups 1 h after reperfusion, compared with the 6 h group (P < 0.05, respectively). Results are mean ± SD for each time point in each group (n = 6). 6 h group—closed triangle, 24 h group— closed square, and 18 h group—closed circle.

Figure 4 Changes in accumulation of neutrophils in the graft after reperfusion, as assessed by MPO/WBC. MPO/WBC in the 24 h (closed square) and 18 h (closed circle) cold-preserved groups increased significantly, compared with the 6 h group (closed triangle) within 0.5 h after reperfusion (P < 0.05, respectively). Results are mean ± SD for each time point in each group (n = 6).

Figure 5 Changes in circulating neutrophil activity in the recipents after reperfusion, as assessed by CD11b and L-selectin expression. A: CD11b expression, B: L-selectin expression. CD11b expression elevated significantly in the 24 h (closed square) and 18 h (closed circle) groups, compared with the 6 h group (closed triangle) 2 h after reperfusion (P < 0.05, respectively). L- selectin shed significantly in the 24 h (closed square) and 18 h (closed circle) groups, compared with the 6 h group (closed triangle) 1 h after reperfusion (P < 0.05, respectively). Results are the mean ± SD for each time point in each group (n = 6).