Transfection efficiency of pORF lacZ plasmid lipopolyplex to hepatocytes and hepatoma cells

Figure 1 Electronic transmission microscopy of LPD (× 35000).

Figure 2 Size distribution of LPD. The particle size was mea-sured by photon correlation spectroscopy (Malvern zetasizer 3000 HS). The average diameter of LPD is 132.1 nm.

Figure 3 Zeta potential of LPD. The zeta potential of LPD was 26.8 mV in 10 mM herps buffer (pH7.4).

Figure 4 Agarose gel electrophoresis of naked DNA and LPD subjected to DNase degradation. Lane 1: LPD incubated with DNase for 5 minutes. Lane 2: LPD incubated with DNase for 1 hour. Lane 3: LPD incubated with DNase for 2 hours. Lane 4: Naked DNA incubated with DNase for 5 minutes. Lane 5: Naked DNA incubated with DNase for 1 hour. Lane 6: Naked DNA incubated with DNase for 2 hours.

Figure 5 pORF LacZ lipopolyplex transfected Chang hepatocytes.

Figure 6 pORF LacZ lipopolyplex transfected SMMC-7721 hepatoma cells.