Modulation of tryptase secretion from human colon mast cells by histamine

Figure 1 Histamine, anti-IgE and calcium ionophore (CI) in-duced tryptase release from colon mast cells. The values shown are mean ± SEM for six separate experiments. Stimulus or HBSS alone was incubated with cells for 15 min before termination of the reactions. ^aP < 0.05 compared with spontaneous release group (paired Student’s t test).

Figure 2 Time course for histamine (100 ng/mL) induced re-lease of tryptase from colon mast cells. Data shown are mean ± SEM of five separate experiments.

Figure 3 Effect of co-addition of histamine (hist) and anti-IgE or calcium ionophore (CI) on tryptase release from colon mast cells. The values shown are mean ± SEM for six separate experiments. Various concentrations of histamine and anti-IgE or CI were added to cells at the same time, and then incubated with cells for 15 min before termination of the reactions. ^aP < 0.05 compared with the corresponding histamine alone group (paired Student’s t test).

Figure 4 Effect of histamine (hist) on anti-IgE or calcium iono-phore (CI) induced tryptase release from colon mast cells. The values shown are mean ± SEM for six separate experiments. Various concentrations of histamine were incubated with cells for 20 min before addition of anti-IgE or CI. ^aP < 0.05 compared with the corresponding histamine alone group (paired Student’s t test). Pred.hist + anti-IgE or CI = the sum of tryptase release induced by histamine alone and anti-IgE or CI alone.