Adenoviral transfer of human interleukin-10 gene in lethal pancreatitis

Figure 1 hIL-10 levels and serum amylase levels in pancreas, liver, and lung after adminstration of AdvIL-10, Adv0, and PBS in healthy and SAP rats. A: hIL-10 levels in pancreas, liver and lung after administration of AdvIL-10, Adv-0 and PBS in healthy rats. B: Serum amylase levels in pancreas, liver and lung after administration of AdvIL-10, Adv-0 and PBS in SAP rats.

Figure 2 Serum amylase levels in heathy rats 24 h after trans-fection with Adv0, PBS and AdvIL-10.

Figure 3 Expression of IL-10 and TNF-α in SAP rat tissues 24 h post- transfection with AdvhIL-10 and PBS. Lanes a, b and c: IL-10 expression in liver, pancreas, and lung, respectively, in AdvIL-10 treated SAP rats; Lanes d, e and f: IL-10 expression in liver, pancreas, and lung, respectively, in the PBS treated SAP rats; Lanes 1, 2, and 3: TNF-α expression in liver, pancreas, and lung, respectively, in PBS treated SAP rats; Lanes 4, 5, and 6: TNF-α expression in liver, pancreas, and lung, respectively, in AdvIL-10 treaed SAP rats. β -actin served as control.

Figure 4 hIL-10 and TNF-α levels in liver, pancreas, and lungs of SAP rats after treatment with AdvIL-10, Adv0, and PBS. A: hIL-10 levels in liver, pancreas and lungs of SAP rats after treatment with AdvIL-10, Adv-0 and PBS. B: TNF-α levels in liver, pancreas and lungs of SAP rats after treatment with Adv-10, Adv-0 and PBS.

Figure 5 Histological sections of rat pancreas (HE, 200 × ). A: Normal rat pancreas; B: Severe necrotizing acute pancreatitis with hemorrhage and necrosis 24 h after induction with sodium taurocholate; C: Acute pancreatitis in rats treated with AdvhIL-10; D: Acute pancreatitis in rats treated with PBS (the pathology changes in the Adv0 group were completely the same as PBS group).

Figure 6 Survival rate of SAP rats after treatment.