Expression of survivin and its significance in colorectal cancer

Figure 1 RT-PCR products of paired samples from normal co-lonic mucosae (lanes 4, 6, 8) and CRC (lanes 3, 5, 7) from three different resection specimens. Lane 1: positive control (U937 cell line) , lane 2: negative control, lane M: DNA mark. Expres-sion of β₂MG confirmed the fidelity of reverse transcription.

Figure 2 Correlations between survivin expression and AI and PI. P < 0.001 as PI in survivin positive tumors (51% ± 22%) compared with survivin negative tumors (27% ± 18%), P < 0.001 as AI in survivin positive tumors (0.67% ± 0.18%) compared with survivin negative tumors (1.14% ± 0.42%).

Figure 3 Immunohistochemical analysis of apoptosis,proliferation,and survivin in CRC. Survivin expression was restricted to the cytoplasm of cancer cells (brown staining, × 400 fold). Apoptotic cells and proliferative cells were detected in the nucleoli of cancer cells (brown staining, × 400 fold).