Effects of herbal compound 861 on human hepatic stellate cell proliferation and activation

Figure 1 Regulation of proliferation in LX-2 and HepG2 cells by Cpd 861. A: Effect of 0.1 mg/mL Cpd 861 on LX-2 cell proliferation. B: Effect of 0.1 mg/mL Cpd 861 on HepG2 cell proliferation. Data are expressed as the mean ± SE of 3 replicates. ^aP < 0.05 vs LX-2 cell controls after 3 d of incubation. ^dP < 0.01 vs LX-2 cell controls after 5 d of incubation. ^fP < 0.01 vs LX-2 cell controls after 7 d of incubation.

Figure 2 Dose dependent effects of Cpd 861 on cell prolifera-tion in LX-2 and HepG2 7 d after incubation. Data are expressed as the mean ± SE of 3 replicates. ^bP < 0.01 vs LX-2 cell controls.

Figure 3 Fluorescence data (A) and standard curve (B) for α-SMA generated on iCycler iQ real-time PCR detection system. Plasmids containing the fragments for α-SMA were diluted from 10⁹ to 10³ copies.

Figure 4 Melting curve for all PCR products after amplification.

Figure 5 Effects of Cpd 861 on expression of α-SMA mRNA in LX-2 cells. LX-2 cells were incubated with 0.01 mg/mL Cpd 861 for different time. The relative number of molecules of α-SMA mRNA after normalization to G3PDH was calculated. The data represent mean ± SE of 4 replicates for each treatment. ^aP < 0.05 vs LX-2 cell controls after 48 h of incubation. ^dP < 0.01 vs LX-2 cell controls after 72 h of incubation.