Inhibitory effect of ubiquitin-proteasome pathway on proliferation of esophageal carcinoma cells

Figure 1 MTT assay of EC9706 cells after exposed to MG-132.

Figure 2 Morphologic changes of EC9706 cells observed un-der microscope after treated with 5 μmol/L of MG-132. A: control group; B: EC9706 cells treated with MG-132 for 24 h; C: EC9706 cells treated with MG-132 for 48 h; D: EC9706 cells treated with MG-132 for 72 h; E: EC9706 cells treated with MG-132 for 96 h (× 200).

Figure 3 Cell cycle and apoptosis of EC9706 cells exposed to 5 μmol/L of MG-132. A: control group. Apoptotic sub-G₁ peak was not found; B: EC9706 cells exposed to MG-132 for 48 h. The ratio of apoptotic cells was 31.7%; C: EC9706 cells ex-posed to MG-132 for 96 h. The ratio of apoptotic cells was 66.4%.

Figure 4 Results of DNA agarose electrophoresis. Lane1: con-trol group; Lane 2: EC9706 cells exposed to 5 μmol/L of MG-132 for 48 h; Lane 3: EC9706 cells exposed to 5 μmol/L of MG-132 for 96 h; Lane 4: 200, 400, 600, 800, 1000, 1200, 1400, 1600, 1800, 2000 bp ladder markers.

Figure 5 Results of immunohistochemical staining of EC9706 cells. A: control group. Immunohistochemical staining of p27^kip1 protein located in cytoplasm of EC9706 cells; B: MG-132 group.