Inhibition of mouse hepatocyte apoptosis via anti-Fas ribozyme

Figure 1 Fas gene transcription in all groups. M: DL-2000 DNA marker, Lanes 1-3: Empty control, mock-transfected group and pU6-RZ596-transfected group.

Figure 2 Fas protein expression in all groups. M: Marker; Lanes 1-3: Empty control, mock-transfected group and pU6-RZ596-transfected group.

Figure 3 Detection of caspase-3 activity in all groups.

Figure 4 Stimulated with JO₂ for 24 h, cells viabilities of control, mock-transfected and pU6-RZ596-transfected groups were detected by MTT assay.

Figure 5 Detection of apoptosis of hepatocytes through cytometry. A: Apoptosis rate of control group was 86%; B: Apoptosis rate of mock-transfected cells was 87%; C: Apoptosis rate of pU6-RZ596-transfected cells was 35%.