Antigen epitope of Helicobacter pylori vacuolating cytotoxin A

doi:10.3748/wjg.v10.i16.2340

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Aug 15, 2004 (publication date) through Apr 29, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 15, 2004; 10(16): 2340-2343
Published online Aug 15, 2004. doi: 10.3748/wjg.v10.i16.2340

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Figure 1 Digestion of recombinant plasmid. M: λ DNA/ Hind III; Lane 1: pLZ-QV3/ Nco I + Hind III; Lane 2: pLZ-QV2/ Nco I + Hind III; Lane 3: pLZ-QV1/ Nco I + Hind III; Lane 4: pQE-60/ Nco I + Hind III.

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Figure 2 SDS-PAGE of LTB-VacA protein. M: Marker; Lane 1: LTB-VacA3; Lane 2: LTB-VacA2; Lane 3: LTB-VacA1; Lane 4: LTB. A: LTB-VacA proteins were expressed in the JM109; B: Purification with anti-LTB antibody affinity chromatography.

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Figure 3 IgG against VacA induced by different LTB-VacA.

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Figure 4 Microscopy of large vacuoles induced by VacA in HeLa cells. A: Normal HeLa cells; B: Vacuolated HeLa cells (Origingal magnification: × 400).

Citation: Liu XL, Li SQ, Liu CJ, Tao HX, Zhang ZS. Antigen epitope of Helicobacter pylori vacuolating cytotoxin A. World J Gastroenterol 2004; 10(16): 2340-2343
URL: https://www.wjgnet.com/1007-9327/full/v10/i16/2340.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i16.2340