Endostatin gene therapy for liver cancer by a recombinant adenovirus delivery

Figure 1 Immunohistochemistry analysis for endostatin pro-tein in vitro (× 100). Extensive positive staining was observed in the cytoplasm of transduced cells. This indicated that human endostatin gene mediated by recombinant adenovirus was highly expressed in BEL-7402 cells.

Figure 2 Effect of Ad/hEndo on the proliferation of HUVEC cells.

Figure 3 Effect of Ad/hEndo on growth of BEL-7402 xenografted liver tumors.

Figure 4 Northern blotting of endostatin mRNA in vivo. Lanes 1-3: RNA extracted from tumors on d 1, 4 and 8 after Ad/hEndo injection, respectively; Lane 4: RNA extracted from control tumors injected with DMEM.

Figure 5 Detection of endostatin concentration in plasma by ELISA.

Figure 6 Pathological changes of liver 1 wk after 6 wk of treatment with Ad/hEndo (HE, original magnification: × 200).