Heme oxygenase-1 in cholecystokinin-octapeptipe attenuated injury of pulmonary artery smooth muscle cells induced by lipopolysaccharide and its signal transduction mechanism

Figure 1 Mitochondria ultrastructural changes shown in EM photograph of PASMCs (×25 000). A: Control group, B: LPS group, C: CCK-8+LPS group, D: CCK-8 group, E: LPS+ZnPP group, F: CCK-8+LPS+ZnPP group.

Figure 2 Effect of CCK-8 on LPS-induced c-fos (upper, ×400) and HO-1 (lower, ×200) protein expression in PASMCs. A: Control group, B: LPS group, C: CCK-8+LPS group, D: CCK-8 group.

Figure 3 RT-PCR product gel electrophoresis. M: DNA marker, 1: Control, 2: LPS, 3: CCK-8 + LPS, 4: CCK-8.

Figure 4 Effect of CCK-8 on LPS-induced HO-1 mRNA ex-pression and JNK activation in PASMCs. A: Effect of CCK-8 on LPS-induced HO-1 mRNA expression in PASMCs. n = 3. ^dP < 0.01 vs Control; ^bP < 0.01 vs LPS. B: CCK-8 increases JNK activation induced by LPS in PASMCs. n = 3. ^aP < 0.05 vs Control; ^bP < 0.01 vs LPS.