Effects of lactose as an inducer on expression of Helicobacter pylori rUreB and rHpaA, and Escherichia coli rLTKA63 and rLTB

Figure 1 Effects of lactose on inducing expression of target recombinant proteins compared to IPTG. A: rUreB; Lane 1: Marker; Lane 2: Induced with 0.5 mmol/L IPTG; Lane 3: In-duced with 100 g/L lactose; Lane 4: Non-induced. B: rHpaA; Lane 1: Marker; Lane 2: Induced with 0.5 mmol/L IPTG; Lane 3: Induced with 50 g/L lactose; Lane 4: Non-induced. C: rLTKA63 expression; Lane 1: Marker; Lane 2: Induced with 0.5 mmol/L IPTG; Lane 3: Induced with 100 g/L lactose. Lane 4: Non-induced; D: rLTB; Lane 1: Marker; Lane 2: Induced with 0.5 mmol/L IPTG; Lane 3: Induced with 100 g/L lactose; Lane 4: Non-induced.

Figure 2 Expression of target recombinant proteins of original bacteria with different A₆₀₀ values under inducement by lactose. ◇: rHpaA; ■: rUreB; ▲: rLTB; □: rLTKA63.

Figure 3 Expression outputs of the target proteins by using different inducing time. ◇: rHpaA; ■: rUreB; ▲: rLTB; □: rLTKA63.