Gene expression profiles in an hepatitis B virus transfected hepatoblastoma cell line and differentially regulated gene expression by interferon-α

Figure 1 RNA slot analyses of IFN-inducible P27, KIAA0919 (Sec15B), beta3gal-T5 and FLJ12673 genes in HepG₂ 2. 2.15 be-fore and after IFN treatment Four genes (interferon related genes P27, KIAA0919, β-3-gal-T5 gene and FLJ12673 human cDNA clone) were selected and tested by RNA slot analysis to confirm the altered expression level indicated by the results of the microarrays.10 μg/sample total cellular RNA was blotted to the nylon membrane. The membrane was then hybridized with [α-³²P]dCTP labeled cDNA probes. Autoradiographs were exposed to X-ray film overnight at -70 °C. The β-actin gene was used as the control to normalize the total RNA quantity.