Blockage of transforming growth factor β receptors prevents progression of pig serum-induced rat liver fibrosis

Figure 1 Primer pairs for PCR reactions.

Figure 2 Enzyme-cutting identification of the recombinant plasmids, Lane 1: pANTI-RII, Lane 2: pANTI-RI, Lane 3: pcDNA3.

Figure 3 Expression of exogenous transfected gene assessed by Northern blot, A: Hybridization using ³²P labeled oligo-nucleotide probe which targets T7 promoter; B: Hybridization by using ³²P labeled TβR II cDNA probe; C: Hybridization by using ³²P labeled TβR I cDNA probe; D: β-actin probe used as control. Lane 1: Antisense TβR II group; Lane 2: pcDNA3 con-trol group; Lane 3: Disease control group; Lane 4: Antisense TβR I group.

Figure 4 Hepatic protein expression of TβR I, TβR II assessed by Western blot, A: Hepatic protein expression of TβR II; B: Hepatic protein expression of TβR I; Lanes 1,8: Disease control group; Lanes 2,7: pcDNA3 control group; Lane 3: Antisense TβR II group; Lanes 4,6: Normal control group; Lane 5: Antisense TβR I group.