Jiang JG, Tang JB, Chen CL, Liu BX, Fu XN, Zhu ZH, Qu W, Cianflone K, Waalkes MP, Wang DW. Expression of cyclooxygenase-2 in human esophageal squamous cell carcinomas. World J Gastroenterol 2004; 10(15): 2168-2173 [PMID: 15259059 DOI: 10.3748/wjg.v10.i15.2168]
Corresponding Author of This Article
Dr. Dao-Wen Wang, Department of Internal Medicine and Gene Therapy Center, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. dwwang@tjh.tjmu.edu.cn
Article-Type of This Article
Esophageal Cancer
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This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Aug 1, 2004; 10(15): 2168-2173 Published online Aug 1, 2004. doi: 10.3748/wjg.v10.i15.2168
Expression of cyclooxygenase-2 in human esophageal squamous cell carcinomas
Jian-Gang Jiang, Jiang-Bo Tang, Chun-Lian Chen, Bao-Xing Liu, Xiang-Ning Fu, Zhi-Hui Zhu, Wei Qu, Katherine Cianflone, Michael P. Waalkes, Dao-Wen Wang
Jian-Gang Jiang, Jiang-Bo Tang, Chun-Lian Chen, Bao-Xing Liu, Xiang-Ning Fu, Zhi-Hui Zhu, Dao-Wen Wang, Internal Medicine Department and Gene Therapy Center, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Wei Qu, Michael P. Waalkes, Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, National Cancer Institute at the National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
Katherine Cianflone, Mike Rosenbloom Laboratory for Cardiovascular Research, McGill University Health Center, Montreal, Quebec H3A 1A1, Canada
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Dao-Wen Wang, Department of Internal Medicine and Gene Therapy Center, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. dwwang@tjh.tjmu.edu.cn
Telephone: +86-27-83662842 Fax: +86-27-83662842
Received: November 17, 2003 Revised: November 23, 2003 Accepted: January 8, 2004 Published online: August 1, 2004
Abstract
AIM: To determine whether cyclooxygenase-2 (COX-2) was expressed in human esophageal squamous cell carcinoma.
METHODS: Quantitative reverse transcription-polymerase chain reaction (RT-PCR), western blotting, immunohistoc-hemistry and immunofluorescence were used to assess the expression level of COX-2 in esophageal tissue.
RESULTS: COX-2 mRNA levels were increased by > 80-fold in esophageal squamous cell carcinoma when compared to adjacent noncancerous tissue. COX-2 protein was present in 21 of 30 cases of esophageal squamous cell carcinoma tissues, but was undetectable in noncancerous tissue. Immunohistochemistry was performed to directly show expression of COX-2 in tumor tissue.
CONCLUSION: These results suggest that COX-2 may be an important factor for esophageal cancer and inhibition of COX-2 may be helpful for prevention and possibly treatment of this cancer.
