MicroRNAs as prognostic biomarkers for survival outcome in osteosarcoma: A meta-analysis

Table 1 Characteristics of eligible studies in this meta-analysis

Ref.	Country	MicroRNA	MicroRNA expression1	Sample size			Specimen	Cut off value	Follow-up (mo)	Outcome	Detection method	Survival analysis	Source of HR	NOS score
				Total	Low	High
Yuan et al[20], 2012	China	miR-21	High	65	NR	NR	Serum	Median	46	OS	RT-PCR	U/M	Reported	6
Ji et al[21], 2013	China	miR-133a	Low	92	46	46	Tissue	Median	60	OS	RT-PCR	U	K-M curve	6
Cai et al[22], 2013	China	miR-210	High	92	44	48	Tissue	Median	100	OS/PFS	RT-PCR	U	K-M curve	6
Yang et al[23], 2013	China	miR-132	Low	166	90	76	Tissue	Median	100	OS/DFS	RT-PCR	U/M	K-M curve	6
Li et al[24], 2014	China	miR-17-92	High	117	45	72	Tissue	Median	60	OS/RFS	RT-PCR	U/M	Reported	6
Xu et al[25], 2014	China	miR-9	High	79	39	40	Tissue	Median	60	OS	RT-PCR	M	Reported	6
Zhang et al[26], 2014	China	miR-133b/ 206	Low	100	NR	NR	Serum	2.7/2.8	40	OS/DFS	RT-PCR	M	K-M curve	8
Cai et al[27], 2014	China	miR-340	Low	92	NR	NR	Tissue	Median	100	OS/PFS	RT-PCR	M	K-M curve	7
Fei et al[28], 2014	China	miR-9	High	118	NR	NR	Serum	Median	80	OS	RT-PCR	U	K-M curve	6
Zhang et al[13], 2014	China	miR-196a/b	High	100	NR	NR	Serum	4.9/5.5	40	OS/DFS	RT-PCR	M	K-M curve	8
Ma et al[29], 2014	China	miR-148a	High	148	NR	NR	Plasma	Median	100	OS/DFS	RT-PCR	U/M	K-M curve	7
Lin et al[30], 2014	China	miR-17-92	High	63	25	38	Tissue	Median	60	OS	RT-PCR	U	K-M curve	6
Song et al[31], 2014	China	miR-26a	Low	144	63	81	Tissue	Median	150	OS/DFS	RT-PCR	M	K-M curve	7
Wang et al[32], 2014	China	miR-214	High	82	33	49	Tissue	Median	60	OS/FFS	RT-PCR	U	K-M curve	7
Cai et al[33], 2015	China	miR-195	Low	166	88	78	Serum	1.44	100	OS/DFS	RT-PCR	M	K-M curve	8
Liu et al[34], 2015	China	miR-126	Low	122	60	62	Tissue	Median	60	OS	RT-PCR	M	Reported	7
Sun et al[35], 2015	China	miR-217	Low	168	84	84	Tissue	Median	80	OS	RT-PCR	U/M	Reported	6
Wang, et al[36], 2015	China	miR-152	Low	80	42	38	Serum	Median	60	OS	RT-PCR	M	Reported	6
Yang et al[37], 2015	China	miR-221	High	108	55	53	Serum	Median	60	OS/RFS	RT-PCR	M	Reported	7
Tang et al[38], 2015	China	miR-27a	High	166	80	86	Serum	Median	100	OS/DFS	RT-PCR	U/M	K-M curve	8
Wang et al[39], 2015	China	miR-191	High	100	42	58	Serum	3.56	60	OS/DFS	RT-PCR	M	K-M curve	8
Cao et al[40], 2016	China	miR-326	Low	60	25	35	Serum	Median	60	OS	RT-PCR	M	Reported	6
Dong et al[41], 2016	China	miR-223	Low	112	53	59	Serum	Median	60	OS	RT-PCR	M	Reported	6
Liu et al[42], 2016	China	miR-300	High	114	46	68	Serum	Median	60	OS/DFS	RT-PCR	U/M	Reported	6
Luo et al[14], 2016	China	miR-125b	Low	56	NR	NR	Plasma	Median	30	OS	RT-PCR	U/M	Reported	7
Niu et al[43], 2016	China	miR-95-3p	Low	133	97	36	Serum	Median	60	OS	RT-PCR	M	Reported	7
Pang et al[44], 2016	China	miR-497	Low	185	91	94	Serum	Median	70	OS	RT-PCR	U/M	Reported	7
Li et al[45], 2016	China	miR-145	low	39	19	20	Tissue	Median	60	OS	RT-PCR	U	K-M curve	7
Zhang and Wang[46], 2016	China	miR-222	High	57	28	29	Serum	Median	80	OS/DFS	RT-PCR	U/M	K-M curve	7
Ren et al[47], 2016	China	miR-21	High	84	33	51	Tissue	Median	100	OS/DFS	RT-PCR	U/M	K-M curve	6
Wang et al[48], 2017	China	miR-34a	low	120	NR	NR	Serum	4.16	60	OS	RT-PCR	M	Reported	7
Yang et al[49], 2017	China	miR-203	Low	103	52	51	Tissue	Median	60	OS/DFS	RT-PCR	U/M	Reported	7
Zhu et al[11], 2017	China	miR-17-5p	High	62	NR	NR	Plasma	Median	70	OS/PFS	RT-PCR	U/M	Reported	6
Nakka et al[50], 2017	United States	miR-221	Low	32	NR	NR	Plasma	Median	150	OS	RT-PCR	M	K-M curve	6
Zou et al[51], 2017	China	miR-19a	High	166	80	86	Tissue	Median	100	OS/DFS	RT-PCR	U/M	Reported	7
Hu et al[52], 2017	China	miR-19a	High	32	NR	NR	Tissue	Median	50	OS	RT-PCR	U/M	Reported	6
Wang et al[53], 2017	China	miR-491	Low	102	58	44	Serum	Median	60	OS	RT-PCR	U/M	Reported	7
Wang et al[54], 2017	China	miR-491-5p	Low	72	36	36	Tissue/ Serum	Median	60	OS/DFS	RT-PCR	M	Reported	6
Tang et al[55], 2017	China	miR-490-3p	Low	148	76	72	Tissue	Median	70	OS	RT-PCR	M	K-M curve	6
Gao et al[56], 2018	China	miR-564	Low	217	107	110	Tissue	Median	60	OS/DFS	RT-PCR	M	K-M curve	7
Yao et al[57], 2018	China	miR-101	Low	152	82	70	Serum	Median	60	OS/RFS	RT-PCR	U/M	K-M curve	6
Cong et al[58], 2018	China	miR-124	Low	114	NR	NR	Serum	Median	60	OS/DFS	RT-PCR	M	Reported	6
Liu et al[59], 2018	China	miR-375	Low	95	47	48	Serum	Median	60	OS	RT-PCR	M	Reported	6
Zhong et al[60], 2018	China	miR-1270	High	143	69	74	Tissue	Median	70	OS	RT-PCR	U/M	Reported	7
Li et al[61], 2018	China	miR-542-3p	High	76	NR	NR	Serum	0.87	70	OS/DFS	RT-PCR	U/M	Reported	8
Zhou et al[62], 2018	China	miR-139-5p	Low	98	53	45	Serum	Median	60	OS	RT-PCR	U/M	Reported	7
Heishima et al[63], 2019	Japan	miR-214	High	106	NR	NR	Plasma	Median	72	OS/DFS	RT-PCR	U/M	Reported	7
Liang et al[12], 2019	China	miR-765	Low	33	NR	NR	Tissue	Median	72	OS	RT-PCR	U/M	Reported	6
Xu, et al[64], 2019	China	miR-106b	High	134	64	70	Tissue	Median	60	OS	RT-PCR	M	Reported	7
Diao et al[65], 2020	China	miR-22	Low	120	60	60	Plasma	Median	60	OS	RT-PCR	U/M	Reported	6
Li et al[66], 2020	China	miR-1826	Low	122	69	53	Tissue	Median	60	OS	RT-PCR	U/M	Reported	7
Li et al[67], 2020	China	miR-629	High	110	45	65	Tissue	Median	60	OS	RT-PCR	M	Reported	7
Yang et al[68], 2020	China	miR-21/ 214	High	63	NR	NR	Tissue	Median	60	OS	RT-PCR	M	Reported	7
Shi et al[69], 2020	China	miR-194	Low	124	69	55	Serum	Median	60	OS/DFS	RT-PCR	M	Reported	7

¹MicroRNA expression with poor prognosis.

High: High expression; Low: Low expression; OS: Overall survival; PFS: Progression-free survival; DFS: Disease-free survival; RFS: Recurrence-free survival; NR: Not reported; M: Multivariate analysis; U: Univariate analysis; NOS: Newcastle-Ottawa scale; RT-PCR: Real-time reverse transcription-PCR.