Impact of metabolism-related mutations on the heart rate of gastric cancer patients after peritoneal lavage

Table 1 Validation of the mutation sites in six patients with a sudden drop in heart rate during distilled water lavage

Mutation type	Chr	Start	End	Ref	Alt	Location	Gene name	FOC	Primers for sequencing (5’-3’)
									Forward	Reverse
SNP
1	chr14	20757383	20757383	A	G	UTR3	TTC5	0/6	CCAAAGAAGAGGAAGGACCTAAAG	CTGTCTCTTCCACTGCTCTCTATGTT
2	chr14	20818983	20818983	C	T	intronic	PARP2-920	0/6	TTTATGAGTTGGCATTCAGAAAGTC	GAAAGGTGAAAGGATTTAGGGAAC
3	chr14	20824859	20824859	T	C	intronic	PARP2-156	0/6	ATAAGAGGGAGTCAGAGGAAGGTGT	AGCACTGAGAACATTACTGTATCCCT
4	chr14	20837701	20837701	G	C	exonic	TEP1-886	0/6	GTGTCTTTAGATGTGGTGTTGGCT	TGGCAGAGTCCTCATTTTTGTGT
5	chr14	20841162	20841162	C	T	intronic	TEP1-0948	0/6	CCTCTAACGCCCAGTAAATACACAG	TCTCTTATCCCAGCAGTATCCCAG
6	chr14	20841313	20841313	G	A	intronic	TEP1-0948	0/6
7	chr14	20841707	20841707	C	T	exonic	TEP1-449	0/6	TATTCCGCATACCTTCCCCAC	CCCTGCTCCGTCCCTTCTT
8	chr16	1756178	1756178	T	C	upstream	MAPK8IP3	0/6	GGAAGCGACCACTGCCG	CATCTGGATCTCCATCATCGC
9	chr17	8161149	8161149	C	T	exonic	PFAS	0/6	GAAAGCCTGTGTAACTCTGATTGC	ATTACTGGCTTCAATGGCAACC
10	chr17	73627539	73627539	T	C	exonic	RECQL5	0/6	GCAATGTCCCTGATGTCCTACC	CTGGAGCAAGACCTGCATCG
11	chr21	35470291	35470291	-	T	UTR3	SLC5A3	0/6	CGAATGTGCTTGTGTGATACTTGTT	GGCAGACTTGTTGGTCATTTCAG
12	chr9	98691137	98691137	T	C	exonic	ERCC6L2	1/6	CTTGATTACCGACGACTTGATGG	ATGTTGATAAAGGAAATGAGAGAAAAGC
Indel
13	chr17	8052526	8052532	AAAAACA	-	intronic	PER1	0/6	TGAAACCCCGTCTCTACTGAAAATAC	TCCCTTAGTGGATTCTTCGCAG
14	chr12	133417982	133417982	-	AA	UTR3	CHFR	1/6	GAAAGGCTTCGTCTCTGCTGC	TGGAGGTGAAGAGAGCGTGTTT

Chr: Chromosome number; Ref: Reference sequence; Alt: Alteration sequence; FOC: Frequency of mutation in the control group; SNP: Single nucleotide polymorphism; Indel: Insertion or deletion; TTC5: Tetratricopeptide repeat domain 5; PARP2: Poly (ADP-ribose) polymerase 2; TEP1: Telomerase associated protein 1; MAPK8IP3: Mitogen-activated protein kinase 8 interacting protein 3; PFAS: Phosphoribosylformylglycinamidine synthase; RECQL5: RecQ like helicase 5; SLC5A3: Solute carrier family 5 member 3; ERCC6L2: ERCC excision repair 6 like 2; PER1: Period circadian regulator 1; CHFR: Checkpoint with forkhead and ring finger domains.