Complete androgen insensitivity syndrome caused by the c.2678C>T mutation in the androgen receptor gene: A case report

Figure 3 Subcellular localization of androgen receptor gene in human embryo kidney 293T and COS7 cells. HEK-293T and COS7 cells were transfected with the fusion protein expression plasmid pEGFP-androgen receptor gene (AR) wild-type (WT), pEGFP-AR P893L, and the pEGFP-NC control plasmid. Twenty-four hours after transfection, cells were treated with 100 nM T. Laser confocal microscope images show that EGFP-AR WT is distributed in the nucleus (D-F, M-O), but that EGFP-AR P893L could not enter the nucleus so has a uniform distribution in the cytoplasm (G-I, P-R).