Identification of the circRNA-miRNA-mRNA regulatory network and its prognostic effect in colorectal cancer

Figure 4 Venn diagrams and heat maps of miRNAs and mRNAs. A: Twenty-five miRNAs were identified from the intersection of 114 differentially expressed (DE) miRNAs with 1602 circRNA targets predicted by the Cancer-Specific CircRNA Database; B: 77 mRNAs were obtained from the intersection of 267 DEmRNAs and 7190 miRNA targets predicted using the TargetScan and miRDB databases; The expression level of each 20 DEmiRNAs (C) and 20 mRNAs (D) expressing the most significant upregulation and downregulation between control tissues (type C) and tumor tissues (type T), respectively. |log₂ fold change (FC)| > 1 and an adjusted P < 0.05 were considered the statistical criteria for DEmiRNA; adjusted P < 0.05 and |log₂ FC| > 2 were considered the statistical criteria for DEmRNA.