Copyright
©The Author(s) 2021.
World J Clin Cases. Apr 16, 2021; 9(11): 2458-2468
Published online Apr 16, 2021. doi: 10.12998/wjcc.v9.i11.2458
Published online Apr 16, 2021. doi: 10.12998/wjcc.v9.i11.2458
Table 1 Polymerase chain reaction steps
| Polymerase chain reaction steps | ||
| First stage | 95℃, 5 min | 1 cycle |
| Second stage | 95℃, 25 s | 15 cycles |
| 64℃, 20 s | ||
| 72℃, 20 s | ||
| Third stage | 93℃, 25 s | 31 cycles |
| 60℃, 35 s | ||
| 72℃, 20 s | ||
- Citation: Fan JZ, Wang GF, Cheng XB, Dong ZH, Chen X, Deng YJ, Song X. Relationship between mismatch repair protein, RAS, BRAF, PIK3CA gene expression and clinicopathological characteristics in elderly colorectal cancer patients. World J Clin Cases 2021; 9(11): 2458-2468
- URL: https://www.wjgnet.com/2307-8960/full/v9/i11/2458.htm
- DOI: https://dx.doi.org/10.12998/wjcc.v9.i11.2458