Sofosbuvir/Ribavirin therapy for patients experiencing failure of ombitasvir/paritaprevir/ritonavir + ribavirin therapy: Two cases report and review of literature

Table 2 Primers used for the amplification of the NS3, NS5A and NS5B regions of the genotype 2a hepatitis C virus genome

Primer name	Sequence (5’ to 3’)1	Nucleotide position2	Notes
HCV/2a-NS3 region
HC686	GTGGARCCYATYATCTTCAGTC	3263-3284	1st sense
HC687	GYACAGCHGGYGGYGTGCTG	3991-4010	RT and 1st antisense
HC688	TYATCTTCAGTCCGATGGAG	3273-3292	2nd sense
HC689	HGGYGGYGTGCTGTTGTCAC	3984-4003	2nd antisense
HCV/2a-NS5A region
HC694	YGCTTCCAGAGGAAACCACG	6118-6137	1st sense
HC695	AGCCCRACGCWRAACGAGAC	6785-6804	RT and 1st antisense
HC696	ACYCAYTACGTGACGGAGTC	6146-6165	2nd sense
HC697	CWRAACGAGACCTCRTCCCG	6776-6795	2nd antisense
HCV/2a-NS5B region
HC673	TGCTGCTCYATGTCWTACTCC	7661-7681	1st sense
HC669	GARTACCTRGTCATRGCCTCC	8686-8706	RT and 1st antisense
HC674	GCTCYATGTCWTACTCCTGGAC	7665-7686	2nd sense
HC670	CATRGCCTCCGTGAAGRCTC	8676-8695	2nd antisense

¹ R=A/G, D=G/A/T, H=A/C/T, W=A/T and Y=T/C.

² Nucleotide positions are numbered in accordance with the HC-J6 strain (D00944) as the HCV/2a reference. HCV: Hepatitis C virus.