Primary leiomyosarcoma of the thyroid gland with prior malignancy and radiotherapy: A case report and review of literature

Table 1 Markers used

Marker	Clone	Source	Antigen retrieval	Dilutions	Visualization kit manufacturer
α SMA	1A4	DAKO	Microwave oven, citrate, pH 6	1:400	DAKO, EnVision
Calponin	EP798Y	Lab Vision	Microwave oven, citrate, pH 6	1:100	DAKO, EnVision
H-caldesmon	h-CALD	LabVision	Water bath, citrate, pH 6	1:300	DAKO, EnVision
CD34	L-END	NOVOCASTRA	Microwave oven, citrate, pH 6	1:100	DAKO, EnVision
p53	DO-7	NOVOCASTRA	Microwave oven, citrate, pH 6	1:50	DAKO, EnVision
Ki67	MIB-1	DAKO	Microwave oven, DAKO 1700, pH 6	1:100	DAKO, EnVision
ER	6F11	NOVOCASTRA	Microwave oven, citrate, pH 6	1:100	DAKO, EnVision
PR	PGR 312	NOVOCASTRA	Microwave oven, citrate, pH 6	1:100	DAKO, EnVision
EBV	CS.1-4	DAKO	Microwave oven, DAKO 1700, pH 6	1:100	DAKO, EnVision

α SMA: Alpha smooth muscle actin; ER: Estrogen receptor; PR: Progesterone receptor; EBV: Epstein-Barr virus.