In-vitro proliferation assay with recycled ascitic cancer cells in malignant pleural mesothelioma: A case report

Figure 3 Collagen gel droplet-embedded culture drug sensitivity test assay of mesothelioma cells obtained from ascites. A: The filtered cancer cells were centrifuged to divide the fluid into a supernatant (i), mucus component (ii), and cell pellet including mesothelioma cells (iii); B: After cell counting, the mesothelioma cells were used to conduct the collagen gel droplet-embedded culture drug sensitivity test (CD-DST). Tumor cells were incubated without any cytotoxic drug; C: Or in the presence of cytotoxic drugs such as D: pemetrexed (PEM) and E: gemcitabine . After the proliferation assay, the final number of cancer cells was quantified using a dedicated system; F: Compared to a normal control, PEM did not reduce cell growth, while gemcitabine reduced the tumor cell growth rate to 21.9%. PEM: Pemetrexed; GEM: Gemcitabine.