Diagnosis of Laron syndrome using monoplex-polymerase chain reaction technology with a whole-genome amplification template: A case report

Figure 1 Electrophoresis of the monoplex polymerase chain reaction assay to identify the deletion of exons 5 and 6 (del5-6) of the growth hormone receptor gene for in vitro fertilization embryos. Lanes E1 to E4 show the whole genome amplification-DNA corresponding to the embryos being analyzed. Lanes L1 to L3 shown the controls previous known to have the deletions. L1 (paternal: heterozygous for del5-6), L2 (affected child: homozygous for del5-6), L3 (maternal: heterozygous for del5-6), masculine (positive control for a homozygous male normal), (-) water control, MWM molecular weight marker (right lane). E1 and E3 show a del5-6, only E1 shows a deletion of ex5, meaning that E1 is homozygous affected, E3 is a heterozygous carrier, and E2 and E4 are wild-types for the deletion causing Laron syndrome. GHR: Growth hormone receptor.