Human podocyte injury in the early course of hypertensive renal injury

Figure 5 Confocal laser scanning micrographs show double immunofluorescence of nephrin–CD2-associated protein under normotensive and hypertensive conditions. Under normotensive conditions, double immunofluorescence staining showed that CD2-associated protein (CD2AP) (A) and nephrin (B) partially colocalized along the basement membrane of glomeruli (C). Under hypertensive conditions, the immunoreactivity of CD2AP and nephrin decreased and stained intermittently (D-F). Scale bar = 20 μm. CD2AP: CD2-associated protein; N: Normotension; HP: Hypertension.