Human podocyte injury in the early course of hypertensive renal injury

Figure 4 Expression of nephrin and CD2-associated protein in human renal tissue by immunohistochemical staining. Tissues were treated with immersion-fixation methods under various hemodynamic conditions. A, C: The localization of nephrin; B, D: The localization of CD2-associated protein; E, F: The integral optical densities of each protein under various hemodynamic conditions. Immunohistochemical localization of both proteins in paraffin-embedded tissue is shown under normotensive (A, B) and hypertensive (C, D) conditions. Scale bar = 20 μm. Histogram shows integral optical density of each protein. ^aP < 0.05, Normotension vs Hypertension. N: Normotension; HP: Hypertension; IODs: Integral optical densities; CD2AP: CD2-associated protein.