Systematic Review
Copyright ©The Author(s) 2019.
World J Clin Cases. Jan 26, 2019; 7(2): 171-190
Published online Jan 26, 2019. doi: 10.12998/wjcc.v7.i2.171
Table 1 Studies included for meta-analysis of exosomal biomarkers in cancer diagnosis
Ref.CountryCancer typeStageControlNumber of ControlNumber of patientsSampleIsolation method of exosomeMarkerDetection methodCut-offTPTNFPFN
Sun et al[35]ChinaColorectalAllHealthy3292PlasmaUCCPNE3ELISA0.143 pg/μg exosome6227530
Ogata-Kawata et al[36]JapanColorectalAllHealthy1188SerumUCmiR-1246qRT-PCR1.45841041
miR-23a0.3100811170
miR-211.085410341
miR-1500.084911390
let-7a0.94410441
miR-2231.724110471
miR-1224-5p0.52811600
miR-12290.062011680
Liu et al[37]ChinaColorectalAllHealthy and benign320148SerumExoQuickCRNDE-hqRT-PCR0.021043021844
Uratani et al[38]JapanColorectalNRHealthy4726SerumExoQuickmiR-21qRT-PCRYouden index183898
Lin et al[39]ChinaGastricAllHealthy6051PlasmaUClncUEGC1qRT-PCRNR4550106
lncUEGC2NR46342617
Zhao et al[40]ChinaGastricAllHealthy120126SerumNRHOTTIPqRT-PCR1.72881021838
Pang et al[41]ChinaGastricAllHealthy3740SerumExoQuickZFAS1qRT-PCRNR322898
Yang et al[42]ChinaGastricAllHealthy8080SerumExoQuickmiR-423-5pqRT-PCRNR65463415
Goto et al[43]JapanPancreaticAllHealthy and advanced pancreatic cancer2223SerumExoQuickmiR-191qRT-PCRDistance = (1-sensitivity)2 + (1-specificity)2 in ROC curve181755
miR-21201843
miR-451a161847
Melo et al[44]GermanyPancreaticAllHealthy100190SerumUCGPC1Flow cytometryYouden index19010000
Que et al[45]ChinaPancreaticAllNon-PDAC2722SerumUCmiR-17-5pqRT-PCR6.826202072
miR-217.693182614
Machida et al[46]JapanPancreatobiliary tractII-IVHealthy1312SalivaTotal exosome isolation kitmiR-1246qRT-PCR13.7781304
miR-4644-5.20591033
Xu et al[47]ChinaLiverAllChronic hepatitis B6888SerumTotal exosome isolation kithnRNPH1qRT-PCR0.6775521613
Sun et al[48]ChinaLiverAllHealthy5656SerumTotal exosome isolation kitLINC00161qRT-PCRNR42411514
Xu et al[49]ChinaLiverAllChronic hepatitis B9660SerumTotal exosome isolation kitENSG00000258332.1qRT-PCR1.34543801617
6055ENSG00000258332.11.36640481215
9660LINC006351.6946752114
6055LINC006351.53244451511
Goldvaser et al[50]IsraelPan-cancer (not include liver)Healthy4598SerumTotal exosome isolation kithTERTqRT-PCRNR6145037
LiverNRHealthy4535NR2145014
Zhang et al[51]ChinaLungAllHealthy3077SerumExoQuickMALAT-1qRT-PCRNR6221915
Sun et al[52]ChinaLungAllHealthy1515PlasmaUC14-3-3ζELISA91236
Li et al[53]NROvarianBenign2150SerumUCephrinA2ELISA20.4 ng/L441746
Meng et al[54]NROvarianAllBenign20163SerumTotal exosome isolation kitmiR-200aPCR+ qRT-PCRYouden index13518228
miR-200b8620077
miR-200c51200112
Pan et al[55]GermanyOvarianAllHealthy29106PlasmaExoQuickmiR-21PCR+ qRT-PCRYouden index6524541
miR-1006621840
miR-200b6825438
miR-3205920947
Bryzgunova et al[56]RussiaProstateAllHealthy2014UrineUCmiR-125qRT-PCRNR121372
miR-19bNR111913
Wang et al[57]ChinaProstateII-IVHealthy3034PlasmaTotal exosome isolation kitSAP30L-AS1qRT-PCRNR2125513
SChLAP1NR302374
Øverbye et al[58]NRProstateAllHealthy1516UrineUCADIRFMass spectrometryYouden index121603
TMEM256141601
Işın et al[59]NRProstateAllBPH4930UrineUrine Exosome RNA Isolation KitLincRNA-p21qRT-PCR0.18120311810
Wang et al[60]ChinaLaryngealAllVocal cord polyps4952SerumExoQuickmiR-21qRT-PCR0.0433640916
HOTAIR0.0324828214
Alegre et al[61]NRMelanomaNRHealthy2553SerumExoQuickexo-MIAELISA1.4 μg/L4220511
exo-S100BELISA0.015 μg/L4220511
Manterola et al[62]FranceGBMNRHealthy3050SerumExoQuickRNU6qRT-PCR0.37233201017
Chen et al[63]TaiwanBladderAllhernia81140UrineUCTACSTD2ELISA2.47 ng/mL103621937
Ge et al[64]ChinaCholangiocarcinomaAllBiliary obstruction5635BileUCENST00000588480.1qRT-PCRNR22411513