Reduced microRNA-451 expression in eutopic endometrium contributes to the pathogenesis of endometriosis

Figure 2 Transfection with miR-451 mimic inhibits cell proliferation by inducing the apoptosis of eutopic cells in endometriosis. A: MiR-451 expression was significantly increased after transfection with miR-451 mimic. Data are expressed as 2^−ΔΔCt (mean ± SEM, n = 4). ^bP < 0.01 vs control and scrambled; B: Cell Counting Kit-8 assays revealed a lower proliferation rate of cells transfected with miR-451 mimic compared to cells in the control and scrambled groups (^aP < 0.05); C and D: Flow cytometric analysis of apoptosis in cells transfected with scrambled siRNA and miR-451 mimic, respectively. Cells are divided into four sections: Q1: Annexin V-FITC- PI+ represents mechanical error; Q2: Annexin V-FITC+ PI+ represents late apoptotic or necrotic cells; Q3: Annexin V-FITC- PI- represents non-apoptotic cells; Q4: Annexin V-FITC+ PI- represents early apoptotic cells.