Isolation and characterization of a new candidate human inactivated rotavirus vaccine strain from hospitalized children in Yunnan, China: 2010-2013

Figure 1 Polyacrylamide gel electrophoresis profiles and VP7 gene sequences of rotavirus strains isolated from diarrhea stools collected in Yunnan, China. A: Electrophoretic migration pattern of RNA from 24 rotavirus-positive stool samples during September 2010 through December 2013. Rotavirus strains Wa and SA11 were used as the markers. The viral RNAs were analyzed by electrophoresis in a 10% polyacrylamide gel and visualized by staining with silver nitrate. L: long electropherotype; S: short electropherotype. Genes 10 and 11 of rotavirus RNA of some samples from September 2012 to December 2013 were not clear in this pattern; B: The partial sequences determined in this study are in bold. The most closely related sequences found in the GenBank database are also included. References for the sequences used in VP7 gene comparisons marked with “G genotype/isolate/country/collected year”. The scale bar represents 5% nucleotide sequence difference. Bootstrap values of > 50% (for 1000 iterations) are shown.