Pan-TRK positive uterine sarcoma in immunohistochemistry without neurotrophic tyrosine receptor kinase gene fusions: A case report

Table 2 Comparison of neurotrophic tyrosine receptor kinase diagnostic tools[14,17]

	Sensitivity	Specificity	Detection of all fusions	Detection of fusion partner	Detection of protein expression	Turnaround time	Material required
IHC	Relatively high (NTRK1 and 2: 75%-96%, NTRK3: 50%-70%)	Relatively high	+	-	+	1 day	1 unstained slide
FISH	High	High	One per probe	One per probe	-	1-3 days	3 unstained slide
RNA NGS	High	High	+	+	+	2-4 weeks	15 unstained slide
DNA NGS	Moderate	High	+	+	-	2-4 weeks	10 unstained slide

NTRK: Neurotrophic tyrosine receptor kinase; NGS: Next-generation sequencing; IHC: Immunohistochemistry; FISH: Fluorescent in situ hybridization.