Inverse relationship between platelet Akt activity and hippocampal atrophy: A pilot case-control study in patients with diabetes mellitus

Figure 1 Phosphorylated-Akt in the platelets. A and B: Type 2 diabetes mellitus (T2DM) group; C and D: The control group. To avoid platelet activation, ice-cold ethylenediaminetetraacetic acid (10 mmol/L) solution was added immediately to the platelet-rich plasma obtained from the patients in the T2DM group (A and B) and the control group (C and D). The lysates of the platelets were then subjected to western blot analysis using antibodies against phospho-specific Akt (Thr-308) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH). All the results are presented collectively (A: n = 40; C: n = 15). The levels of GAPDH and phosphorylated Akt were determined using the ImageJ software program. The bar graphs show the levels of phosphorylated Akt corrected by those of GAPDH in the T2DM group (B) and the control group (D). GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.