Multi-organ hereditary hemorrhagic telangiectasia: A case report

Figure 5 The arginine to glutamate substitution at amino acid position 374 mutation affects ALK1 kinase activity. A: Influence of ALK1 on Smad1 phosphorylation was investigated by immunoblotting. Protein levels of Smad1 and phosphorylated Smad1 (P-Smad1) were detected in HepG2, Hela, and 293T cell lines; B: Kinase activity of ALK1 was detected in the wildtype (WT) group and the mutant group through the BRE4 reporter luciferase assay (^aP < 0.05 and ^bP < 0.01 vs the wildtype group); C: Protein level of collagen-1 was detected by immunoblotting in HepG2, Hela, and 293T cell lines. R374Q: Arginine to glutamate substitution at amino acid position 374. WT: Wildtype.