High tumor mutation burden indicates a poor prognosis in patients with intrahepatic cholangiocarcinoma

Table 3 Univariable and multivariable analysis of overall survival

	Univariable			Multivariable
	HR	95%CI	P value	HR	95%CI	P value
Age, yr
< 65
≥ 65	0.96	0.76-1.21	0.7286	1.16	0.84-1.61	0.3662
Gender
Female
Male	1.27	1.00-1.61	0.0424	1.27	0.94-1.72	0.1145
BMI
< 28
≥ 28	0.80	0.63-1.00	0.0536	0.84	0.62-1.132	0.2517
TMB, mut/Mb
Low (≤ 3.1)
High (> 3.1)	1.47	1.13-1.91	0.002	1.43	1.05-1.96	0.0240
CA19-9
< 40 U/mL
≥ 40 U/mL	1.79	1.37-2.33	< 0.0001	1.78	1.28-2.46	0.0005
Chronic viral hepatitis
Negative
Positive	1.19	0.78-1.83	0.3667	1.72	1.01-2.95	0.0468
Tumor resection
Resected
Unresected	3.09	2.39-3.99	< 0.0001	2.58	1.72-3.88	< 0.0001
Tumor grade
Well differentiated
Moderately differentiated	2.01	1.19-3.39	0.0471	1.08	0.49-2.38	0.8489
Poorly differentiated	2.26	1.34-3.80	0.0211	1.15	0.52-2.57	0.7298
Disease progression
Solitary liver tumor
Multifocal liver disease	2.26	1.53-3.36	< 0.0001	1.47	0.86-2.52	0.1587
Metastatic disease	3.79	2.87-5.01	< 0.0001	2.55	1.55-4.20	0.0002
Smoking status
Never smoked
Former smoker	1.02	0.80-1.31	0.8591	0.94	0.69-1.29	0.7077
Current smoker	1.03	0.69-1.52	0.8971	1.64	0.98-2.73	0.0587

BMI: Body mass index; TMB: Tumor mutation burden; CI: Confidence interval.