Myeloproliferative neoplasms complicated with β-thalassemia: Two case report

Figure 2 Bone marrow smear, biopsy section, Karyotype, Partial sequencing electropherogram and polymerase chain reaction and reverse dot blot observed in this case. A: Hematoxylin and eosin staining (1000×, 100×) of the bone marrow aspirate showed teardrop-shaped, oval, long red blood cells, active hyperplasia of bone marrow granulocytes, erythroblastic cells that were hypoplastic, and a normal number of megakaryocytes with a decreased platelet production; B: PCR-based reverse dot blot showing β-IVS-II-654-thalassemia; C: Karyotype: 46 chromosomes, including XY, t(11; 18)(q13; p11.2), and ins(17; 17)(q21; q25q12)[20]; D: Partial sequencing electropherogram showing a type 1 (49-bp) deletion in exon 9 of CALR (194-242, GAG……. TGT); E: Hematoxylin and eosin staining (100×, 200×) showing BM reticulin (+++) with megakaryocyte hyperplasia and dysplasia.