Comprehensive proteomic signature and identification of CDKN2A as a promising prognostic biomarker and therapeutic target of colorectal cancer

Figure 1 Quantitative proteomic profiling and bioinformatic analysis of colorectal cancer. A: Partial least squares-discriminant analysis of colorectal cancer and normal tissues; B: The volcano plot shows 1115 upregulated and 705 downregulated proteins in tumors; C: Gene ontology analysis shows that differentially expressed proteins are involved in biological processes, cellular components, and molecular functions; D: Kyoto encyclopedia of genes and genomes analysis shows that differentially expressed proteins are significantly enriched in multiple pathways including ribosome biogenesis in eukaryotes, focal adhesion, cell cycle and extracellular matrix-receptor interaction; E and F: Gene set enrichment analysis shows that differentially expressed proteins are enriched in the cell cycle and p53 signaling pathway. PLS-DA: Partial least squares-discriminant analysis; CRC: Colorectal cancer tissue; CN: Normal colon tissue; GO: Gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; ECM: Extracellular matrix; NES: Normalized enrichment score.