Clinical and Translational Research
Copyright ©The Author(s) 2022.
World J Clin Cases. Jun 26, 2022; 10(18): 5965-5983
Published online Jun 26, 2022. doi: 10.12998/wjcc.v10.i18.5965
Table 5 Primer sequence in the real-time polymerase chain reaction
Primer name
Primer sequence, 5’ to 3’
GAPDH-F (internal reference)5-GGAGCGAGATCCCTCCAAAAT-3
GAPDH-R (internal reference)5-GGCTGTTGTCATACTTCTCATGG-3
ACTB-F (internal reference)5-CATGTACGTTGCTATCCAGGC-3
ACTB-R (internal reference)5-CTCCTTAATGTCACGCACGAT-3
IL-1B-F5-TTCGACACATGGGATAACGAGG-3
IL-1B-R5-TTTTTGCTGTGAGTCCCGGAG-3
CXCL9-F5-CCAGTAGTGAGAAAGGGTCGC-3
CXCL9-R5-AGGGCTTGGGGCAAATTGTT-3
CCL8-F5-TGGAGAGCTACACAAGAATCACC-3
CCL8-R5-TGGTCCAGATGCTTCATGGAA-3
CLDN8-F5-CTACAGGCAGCCAGAGGACT-3
CLDN8-R5-ACAGGGATGAGCACCACCAT-3
hsa-U6 (internal reference)
hsa-miR-30a-3p5-CTTTCAGTCGGATGTTTGCAGC-3
hsa-miR-181a-5p5-AACATTCAACGCTGTCGGTGAGT-3
hsa-miR-21-5p5-TAGCTTATCAGACTGATGTTGA-3