Identification of circ_0000375 and circ_0011536 as novel diagnostic biomarkers of colorectal cancer

Figure 5 Circ_0000375 and circ_0011536 serve as sponges of miR-1182 and miR-1246 in colorectal cancer. A: The expression level of miR-1182 in a normal colon cell line (FHC) and colorectal cancer cell lines (HCT116, RKO and SW480); B: The relative expression level of miR-1182 in SW480 and HCT116 cells transfected with a circ_0000375 overexpression vector; C: Relative luciferase activities were detected in HEK293T cells after cotransfection with circ_0000375-WT, circ_0000375-MUT, miR-1182 mimics and negative control mimics; D: The expression level of miR-1246 in colorectal cancer cell lines; E: The relative expression level of miR-1246 in SW480 and HCT116 cells transfected with oe-circ_0011536; F: Luciferase reporter assays were performed after cotransfection with circ_0011536-WT, circ_0011536-MUT, miR-1246 mimics and negative control mimics. ^aP < 0.001; ^bP < 0.01; ^cP < 0.05. NC: Negative control; NS: Not significant.