DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis

doi:10.5662/wjm.v9.i3.32

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Nov 14, 2019 (publication date) through Dec 4, 2024

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World Journal of Methodology

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2222-0682

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Methodol. Nov 14, 2019; 9(3): 32-43
Published online Nov 14, 2019. doi: 10.5662/wjm.v9.i3.32

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Figure 1 Workflow for extraction of DNA from archived H&E-stained cancer tissue slides. A: Slides selected for the extraction of DNA; B: Coverslip removal and scraping of the tissues; C: Xylene treatment for deparaffinization; D: Ethanol rehydration and stain removal; E: Tissue digestion; F: Nucleic acid purification by phase separation followed by DNA precipitation; G: Quantification of DNA using Nanodrop; H: Assessment of quality of the extracted DNA.

Citation: Ramesh PS, Madegowda V, Kumar S, Narasimha S, S R P, Manoli NN, Devegowda D. DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis. World J Methodol 2019; 9(3): 32-43
URL: https://www.wjgnet.com/2222-0682/full/v9/i3/32.htm
DOI: https://dx.doi.org/10.5662/wjm.v9.i3.32