Bifunctional staining for ex vivo determination of area at risk in rabbits with reperfused myocardial infarction

Figure 2 Double suture method in rabbit model of reperfused myocardial infarction. A: A 1/2 circle shape triangular needle has spring eyes at the end; B: Two silk sutures were placed through separate eyes; C: The thicker suture (2-0) was used for the left circumflex coronary artery (LCx) ligation, which would be removed for reperfusion, and the thinner one (5-0) was spared for later ex vivo LCx re-occlusion in order to perform postmortem bifunctional staining.