LC-MS/MS analysis of 2-aminothiazoline-4-carboxylic acid as a forensic biomarker for cyanide poisoning

Figure 6 2-aminothiazoline-4-carboxylic acid concentration in mice plasma after different doses of potassium cyanide (endogenous level = 0 mg/kg body weight dose of potassium cyanide). Mice received three different sublethal doses (6, 8 and 10 mg/kg) of cyanide subcutaneously (3 mice/doses) and were terminated 15 min after cyanide exposure. A significant correlation of cyanide dose vs 2-aminothiazoline-4-carboxylic acid (ATCA) concentration in plasma samples was observed. ATCA concentration in mice plasma samples was increased from 189 ± 28 ng/mL (n = 3 mice) for endogenous level [0 mg/kg body weight dose of potassium cyanide (KCN)] to 413 ± 66 ng/mL (n = 3 mice) for 10 mg/kg body weight dose of KCN.